Jones Les P, Zheng Hao-Qiang, Karron Ruth A, Peret Teresa C T, Tsou Cecelia, Anderson Larry J
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Clin Diagn Lab Immunol. 2002 May;9(3):633-8. doi: 10.1128/cdli.9.3.633-638.2002.
The role of strain differences in respiratory syncytial virus (RSV) disease has not been clearly defined. To investigate the possibility that strain differences contribute to susceptibility to repeat infections, we developed assays to detect antibodies to the two variable regions of the RSV G protein by cloning and expressing the internal variable region at amino acids (aa) 60 to 172 (g1) and the carboxy-terminal variable region at aa 193 to the carboxy terminus (g2) from different genotypes of RSV. The purified proteins were covalently linked to beads with different proportions of red and orange fluorescent dyes and reacted against serum specimens. Antibody reacting against the differently colored beads, and thus against different G polypeptides, was detected by use of flow cytometry and the Luminex system. This assay system detected group- and, to some extent, genotype-specific responses to RSV infection and can be used to investigate the role of strain differences in RSV disease.
呼吸道合胞病毒（RSV）疾病中毒株差异的作用尚未明确界定。为了研究毒株差异是否会导致重复感染易感性，我们通过克隆和表达来自不同基因型RSV的位于氨基酸（aa）60至172的内部可变区（g1）以及位于aa 193至羧基末端的羧基末端可变区（g2），开发了检测针对RSV G蛋白两个可变区抗体的检测方法。将纯化的蛋白与含有不同比例红色和橙色荧光染料的珠子共价连接，并与血清标本反应。通过流式细胞术和Luminex系统检测与不同颜色珠子反应、从而与不同G多肽反应的抗体。该检测系统可检测针对RSV感染的组特异性以及在一定程度上的基因型特异性反应，可用于研究毒株差异在RSV疾病中的作用。
