[肾移植受者巨细胞病毒感染的检测]
[Detection of cytomegalovirus infection in recipients of renal transplantation].
作者信息
Fan J, Qian J, He Q, Ma W H, Chen J H, Zhou L, Cai T
机构信息
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2001 Sep;15(3):262-4.
OBJECTIVE
To investigate infection of cytomegalovirus (CMV) in recipients of renal transplantation (RT).
METHODS
167 recipients of RT were screened for the occurrence of CMV infection by using ELISA to test anti-CMV IgG, IgM from serum samples, using immunohistochemistry method to test CMV antigen expression and nest PCR to amplify CMV DNA from blood samples.
RESULTS
Of these samples, 165 and 3 came out positive of IgG and IgM with the positive rate being 98.8% and 1.8% respectively. 79 samples resulted in antigen expression (47.3%) with the average antigen index being (3.2, 3.1)/5, 104 WBC. Besides, 85 samples were found to be positive by nest-PCR with the positive rate being 50.9%.
CONCLUSIONS
The results indicate that the infection of RT so the detection CMV is common in recipients of RT. So detection of CMV-antibody, CMV antigen and CMV DNA after renal transplantation is very important in early diagnosis of CMV infection.
目的
探讨肾移植受者巨细胞病毒（CMV）感染情况。
方法
采用酶联免疫吸附测定法（ELISA）检测167例肾移植受者血清样本中的抗CMV IgG、IgM，采用免疫组织化学方法检测CMV抗原表达，采用巢式聚合酶链反应（PCR）从血液样本中扩增CMV DNA，以筛查CMV感染的发生情况。
结果
这些样本中，IgG阳性165例，IgM阳性3例，阳性率分别为98.8%和1.8%。79例样本出现抗原表达（47.3%），平均抗原指数为（3.2，3.1）/5，104白细胞。此外，85例样本经巢式PCR检测为阳性，阳性率为50.9%。
结论
结果表明肾移植受者中CMV感染情况及CMV检测较为常见。因此，肾移植后检测CMV抗体、CMV抗原和CMV DNA对CMV感染的早期诊断非常重要。
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