Ortega H H, Joazeiro P P, Muñoz-de-Toro M M, Luque E H, Montes G S
Department of Human Physiology, Faculty of Biochemistry and Biological Sciences, UNL, Santa Fe, Argentina.
J Submicrosc Cytol Pathol. 2001 Oct;33(4):463-72.
The pubic joint of male and female rats was studied at the light- and electron microscopical levels using methods that selectively disclose the extracellular matrix fibres and glycosaminoglycans. The interpubic tissue showed no difference between sexes (including pregnant and intrapartum females). The medial ends of the pubic bones were covered by articular caps of hyaline cartilage that blended in the midline. The whole articular cartilage was covered dorsally and ventrally (as well as craneally and caudally) by a typical perichondrium. The differential distribution of the fibres of the collagenous and elastic systems in the pubic joint agreed with the results reported in the literature for other rat cartilages. Collagen fibres, composed mainly of type-I collagen, were localised to the fibrous perichondrium and bone. Type-II collagen was localised to the central nucleus of hyaline cartilage, whereas reticulin fibres (rich in type-III collagen) were found in the adventitial loose connective tissue adherent to the most superficial layer of the perichondrium. The central nucleus of hyaline cartilage possessed the two types of elastic-related fibres: elaunin fibres were localised mainly to the chondrogenic layer of the perichondrium, whereas oxytalan fibres were found in the matrix that surrounded the chondrocytes. The bulk of the glycosaminoglycans present in the pubic joint cartilage corresponded to hyaluronic acid and chondroitin sulphate. The propriety of classification of the rat pubic joint as a true synchondrosis (instead of symphysis), and the fact that the unaltered pelvis of the rat seems to be adequate for normal parturition, are discussed.
采用选择性揭示细胞外基质纤维和糖胺聚糖的方法，在光镜和电镜水平对雄性和雌性大鼠的耻骨联合进行了研究。耻骨间组织在性别之间（包括怀孕和分娩期的雌性）没有差异。耻骨的内侧端被透明软骨的关节囊覆盖，关节囊在中线处融合。整个关节软骨在背侧和腹侧（以及头侧和尾侧）被一层典型的软骨膜覆盖。耻骨联合中胶原和弹性系统纤维的差异分布与文献中报道的其他大鼠软骨的结果一致。主要由I型胶原组成的胶原纤维定位于纤维软骨膜和骨。II型胶原定位于透明软骨的中央核，而网状纤维（富含III型胶原）则存在于附着于软骨膜最表层的外膜疏松结缔组织中。透明软骨的中央核有两种与弹性相关的纤维：弹力纤维主要定位于软骨膜的软骨生成层，而氧化弹性纤维则存在于围绕软骨细胞的基质中。耻骨联合软骨中存在的大部分糖胺聚糖对应于透明质酸和硫酸软骨素。本文讨论了将大鼠耻骨联合归类为真正的软骨结合（而非耻骨联合）的合理性，以及大鼠未改变的骨盆似乎足以进行正常分娩这一事实。
