Andrades-Miranda Jaqueline, Zanchin Nilson I T, Oliveira Luiz F B, Langguth Alfredo R, Mattevi Margarete S
Departamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Genetica. 2002;114(1):11-6. doi: 10.1023/a:1014645731798.
Chromosome preparations of 30 specimens of Oryzomys subflavus trapped in eight Brazilian localities were C-, and G-banded and analyzed by fluorescence in situ hybridization (FISH). Two karyotypes were found, 2n = 50/FN = 64, at three coastal localities of the Atlantic Forest domain, and 2n = 58/FN = 70 at two sites located in the Cerrado biome, Brazil Central. Two fluorescence in situ hybridization (FISH) patterns of the telomeric sequence (T2AG3)n were observed: in both karyotypes the probes hybridized to the telomeres of all chromosomes and also a hybridization signal in the centromeric regions of two autosome pairs was seen in the 2n = 50 karyotype. These results, together with the occurrence of other diploid numbers described in the literature, suggest that 0. subflavus is a complex species, bearing fusion/fission rearrangements proper to the different biomes which it inhabits.
对在巴西八个地区捕获的30只黄腹稻鼠（Oryzomys subflavus）标本进行了染色体标本制备，进行C带和G带处理，并通过荧光原位杂交（FISH）分析。在大西洋森林区域的三个沿海地区发现了两种核型，2n = 50/FN = 64，在巴西中部塞拉多生物群落的两个地点发现了2n = 58/FN = 70。观察到端粒序列（T2AG3）n的两种荧光原位杂交（FISH）模式：在两种核型中，探针均与所有染色体的端粒杂交，并且在2n = 50的核型中，在两对常染色体的着丝粒区域也观察到杂交信号。这些结果，连同文献中描述的其他二倍体数目的出现，表明黄腹稻鼠是一个复杂的物种，具有适合其栖息的不同生物群落的融合/分裂重排。
