有机溶剂对木瓜蛋白酶动力学的影响。
Influence of organic solvents on papain kinetics.
作者信息
Szabelski M, Stachowiak K, Wiczk W
机构信息
Faculty of Chemistry, University of Gdańsk, Poland.
出版信息
Acta Biochim Pol. 2001;48(4):1197-201.
PMID:11995992
Abstract
Papain activity was studied in water-organic solvent mixtures using the fluorogenic substrate Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans. The increase of organic solvent (MeOH, EtOH, iPrOH, TFE, MeCN, (MeO)2Et and DMF) concentration in the mixture caused a substantial decrease the initial rate of papain-catalyzed hydrolysis. Moreover, the number of papain active sites decreased with the increase of DMF and MeOH concentration.
摘要
使用荧光底物Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans在水-有机溶剂混合物中研究了木瓜蛋白酶的活性。混合物中有机溶剂（甲醇、乙醇、异丙醇、三氟乙醇、乙腈、乙二醇二甲醚和二甲基甲酰胺）浓度的增加导致木瓜蛋白酶催化水解的初始速率大幅下降。此外，木瓜蛋白酶活性位点的数量随着二甲基甲酰胺和甲醇浓度的增加而减少。
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