Texier A C, Andrès Y, Faur-Brasquet C, Le Cloirec P
Ecole des Mines de Nantes, GEPEA, France.
Chemosphere. 2002 Apr;47(3):333-42. doi: 10.1016/s0045-6535(01)00244-2.
A fixed-bed study was carried out by using cells of Pseudomonas aeruginosa immobilized in polyacrylamide gel as a biosorbent for the removal of lanthanide (La, Eu, Yb) ions from aqueous solutions. The effects of superficial liquid velocity based on empty column, particle size, influent concentration and bed depth on the lanthanum breakthrough curves were investigated. Immobilized biomass effectively removed lanthanum from a 6 mM solution with a maximum adsorption capacity of 342 micromolg(-1) (+/-10%) corresponding closely to that observed in earlier batch studies with free bacterial cells. The Bohart and Adams sorption model was employed to determine characteristic parameters useful for process design. Results indicated that the immobilized cells of P. aeruginosa enable removal of lanthanum, europium and ytterbium ions from aqueous effluents with significant and similar maximum adsorption capacities. Experiments with a mixed cation solution showed that the sequence of preferential biosorption was Eu3+ > or = Yb3+ > La3+. Around 96+/-4% of the bound lanthanum was desorbed from the column and concentrated by eluting with a 0.1 M EDTA solution. The feasibility of regenerating and reusing the biomass through three adsorption/desorption cycles was suggested. Neural networks were used to model breakthrough curves performed in the dynamic process. The ability of this statistical tool to predict the breakthrough times was discussed.
采用固定在聚丙烯酰胺凝胶中的铜绿假单胞菌细胞作为生物吸附剂，从水溶液中去除镧系元素（La、Eu、Yb）离子，进行了固定床研究。研究了基于空柱的表面液体流速、粒径、进水浓度和床层深度对镧穿透曲线的影响。固定化生物质有效地从6 mM溶液中去除了镧，最大吸附容量为342 μmol g(-1)（±10%），这与早期游离细菌细胞的批次研究中观察到的结果非常接近。采用Bohart和Adams吸附模型来确定对工艺设计有用的特征参数。结果表明，铜绿假单胞菌的固定化细胞能够从水性流出物中去除镧、铕和镱离子，具有显著且相似的最大吸附容量。混合阳离子溶液的实验表明，优先生物吸附顺序为Eu3+ ≥ Yb3+ > La3+。通过用0.1 M EDTA溶液洗脱，约96±4%的结合镧从柱中解吸并浓缩。提出了通过三个吸附/解吸循环对生物质进行再生和再利用的可行性。利用神经网络对动态过程中的穿透曲线进行建模。讨论了这种统计工具预测穿透时间的能力。
