Mérigout Patricia, Képès François, Perret Anne-Marie, Satiat-Jeunemaitre Béatrice, Moreau Patrick
Laboratoire de Dynamique de la Compartimentation Cellulaire, Institut des Sciences du Végétal, CNRS UPR 2355, 91198 Cedex, Gif-sur-Yvette, France.
FEBS Lett. 2002 May 8;518(1-3):88-92. doi: 10.1016/s0014-5793(02)02651-0.
Effects of brefeldin A (BFA) and nordihydroguaiaretic acid (NDGA) on endomembrane structures and lipid synthesis were compared in maize root cells and tobacco Bright Yellow-2 cells. Immunofluorescence and electron microscopy studies showed that NDGA altered the structure and distribution of the endoplasmic reticulum (ER) within 1 h but not of the Golgi apparatus whereas, as shown previously, BFA altered that organization of the Golgi apparatus and, only subsequently, of the ER. Biochemical studies revealed that both drugs and especially BFA led to a strong inhibition of the phytosterol biosynthetic pathway: BFA led to accumulation of sterol precursors. The importance of phytosterols in membrane architecture and membrane trafficking is discussed.
在玉米根细胞和烟草亮黄-2细胞中比较了布雷菲德菌素A（BFA）和去甲二氢愈创木酸（NDGA）对内膜结构和脂质合成的影响。免疫荧光和电子显微镜研究表明，NDGA在1小时内改变了内质网（ER）的结构和分布，但未改变高尔基体的结构和分布，而如先前所示，BFA改变了高尔基体的组织结构，随后才改变内质网的组织结构。生化研究表明，这两种药物，尤其是BFA，强烈抑制了植物甾醇生物合成途径：BFA导致甾醇前体的积累。文中讨论了植物甾醇在膜结构和膜运输中的重要性。
