Yamamoto Hiroaki, Matsuyama Akinobu, Kobayashi Yoshinori
Tsukuba Research Center, Daicel Chemical Industries, Ltd., Ibaraki, Japan.
Biosci Biotechnol Biochem. 2002 Feb;66(2):481-3. doi: 10.1271/bbb.66.481.
The synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate ((R)-ECHB) from ethyl 4-chloroacetoacetate was studied using whole recombinant cells of Escherichia coli expressing a secondary alcohol dehydrogenase of Candida parapsilosis. Using 2-propanol as an energy source to regenerate NADH, the yield of (R)-ECHB reached 36.6 g/l (more than 99% ee, 95.2% conversion yield) without addition of NADH to the reaction mixture.
利用表达近平滑假丝酵母仲醇脱氢酶的大肠杆菌全重组细胞，研究了由4-氯乙酰乙酸乙酯合成(R)-4-氯-3-羟基丁酸乙酯((R)-ECHB)的过程。以2-丙醇作为能量来源来再生NADH，在不向反应混合物中添加NADH的情况下，(R)-ECHB的产量达到36.6 g/L（对映体过量率超过99%，转化率为95.2%）。
