Hansen J T, Yates R D, Chen I L
Am J Anat. 1975 Dec;144(4):491-502. doi: 10.1002/aja.1001440409.
Young male and female New Zealand white rabbits were given a daily subcutaneous injection of reserpine (Serpasil, Ciba; 3 mg/kg) for two days and were sacrificed 24 hours after the last injection. The subclavian glomera (aortic bodies) were processed for electron microscopy to determine the effects of this biogenic amine depleting agent on the electron-opaque cytoplasmic granules of the parenchymal type I cells. Observations of glutaraldehyde-osmium tetroxide fixed glomera from reserpinized animals showed a slight decrease in granule density of the type I cells. Glomera fixed in glutaraldehyde and incubated in potassium dichromate (pH 4.1) demonstrated a reduction in granule opacity following reserpine treatment. Control glomera incubated in potassium dichromate displayed electron-opaque granules. These results indicate that reserpine does deplete the amines without granule disappearance or changes in granule population. The positive reaction of the control tissue granules to potassium dichromate incubation suggests that the predominant biogenic amines in the electron-opaque granules are unsubstituted monoamines. Persistence of the opaque granules following reserpinization and glutaraldehyde-osmium tetroxide double fixation, may be due to amine-binding protein within the granules. The mode of granule depletion could not be ascertained with certainty.
给年轻的雄性和雌性新西兰白兔每日皮下注射利血平（蛇根碱，汽巴公司生产；3毫克/千克），持续两天，并在最后一次注射后24小时处死。对锁骨下肾小球（主动脉体）进行电子显微镜处理，以确定这种生物胺耗竭剂对实质I型细胞电子不透明细胞质颗粒的影响。对来自利血平化动物的戊二醛-四氧化锇固定的肾小球的观察显示，I型细胞的颗粒密度略有降低。在戊二醛中固定并在重铬酸钾（pH 4.1）中孵育的肾小球显示，利血平处理后颗粒不透明度降低。在重铬酸钾中孵育的对照肾小球显示出电子不透明颗粒。这些结果表明，利血平确实能消耗胺类，而不会导致颗粒消失或颗粒数量改变。对照组织颗粒对重铬酸钾孵育的阳性反应表明，电子不透明颗粒中的主要生物胺是未取代的单胺。利血平化和戊二醛-四氧化锇双重固定后不透明颗粒的持续存在，可能是由于颗粒内的胺结合蛋白。颗粒消耗的方式尚不能确定。
