多形性胶质母细胞瘤患者p16基因纯合缺失分析及其与生存的相关性

Analysis of homozygous deletion of the p16 gene and correlation with survival in patients with glioblastoma multiforme.

作者信息

Kamiryo Takanori, Tada Kenji, Shiraishi Shoji, Shinojima Naoki, Nakamura Hideo, Kochi Masato, Kuratsu Jun-ichi, Saya Hideyuki, Ushio Yukitaka

机构信息

Department of Neurosurgery, Kumamoto University Medical School, Japan.

出版信息

J Neurosurg. 2002 May;96(5):815-22. doi: 10.3171/jns.2002.96.5.0815.

DOI:10.3171/jns.2002.96.5.0815

PMID:12005388

Abstract

OBJECT

One of the most frequent genetic abnormalities found in patients with glioblastoma multiforme (GBM) is homozygous deletion of the p16 tumor suppressor gene. The authors investigated whether this deletion is associated with prognosis in patients with GBM.

METHODS

In 46 adult patients with supratentorial GBM, homozygous deletion of the p16 gene in tumor DNA was examined using the multiplex polymerase chain reaction assay. The deletion was confirmed in 14 (30.4%) of 46 patients, eight (30.8%) of 26 men and six (30.0%) of 20 women. Cox proportional hazard regression analysis, adjusted for age at surgery, the Karnofsky Performance Scale score, extent of resection, and the MIB-1 labeling index. revealed that homozygous deletion of the p16 gene was significantly associated with overall survival and progression-free survival in men, but not in women.

CONCLUSIONS

The results of this study suggest that p16 homozygous deletion is a significant unfavorable prognostic factor in male patients with GBM.

摘要

目的

在多形性胶质母细胞瘤（GBM）患者中发现的最常见的基因异常之一是p16肿瘤抑制基因的纯合缺失。作者研究了这种缺失是否与GBM患者的预后相关。

方法

对46例幕上GBM成年患者，采用多重聚合酶链反应检测肿瘤DNA中p16基因的纯合缺失。46例患者中有14例（30.4%）证实存在缺失，其中男性26例中有8例（30.8%），女性20例中有6例（30.0%）。Cox比例风险回归分析对手术年龄、卡氏功能状态评分、切除范围和MIB-1标记指数进行了校正。结果显示，p16基因的纯合缺失与男性患者的总生存期和无进展生存期显著相关，但与女性患者无关。