Chen Hsing-Chen, Chang Chiao-Ching, Mau Wei-Juin, Yen Lin-Shiun
Department of Food Science, National Taiwan Ocean University, Keelung 202, Taiwan.
FEMS Microbiol Lett. 2002 Mar 19;209(1):53-6. doi: 10.1111/j.1574-6968.2002.tb11108.x.
N-Acetylchitooligosaccharides ((GlcNAc)(n)) with different degrees of polymerization (n=1-6) were prepared as the main carbon sources in media for evaluating the growth of nine intestinal bacteria. A chitohydrolysate was prepared by hydrolyzing shrimp-shell chitin using HCl. After purification, the purity of each (GlcNAc)(1-6) was >86%. The growth of intestinal bacteria was carried out in a basal medium (BM) containing 0.2% (w/v) of each sugar or glucose as the main carbon source and was evaluated using maximum cell densities and specific growth rates. Bacteroides fragilis and Clostridium perfringens could respectively utilize GlcNAc and (GlcNAc)(2) more efficiently for growth than glucose. Bifidobacterium adolescentis and Eubacterium limosum could use (GlcNAc)(1-6) slightly as their main carbon source. Escherichia coli, Lactococcus lactis and Proteus vulgaris could utilize glucose more efficiently than (GlcNac)(1-6). GlcNAc was used more readily than (GlcNAc)(2-6) by Staphylococcus aureus, exhibiting almost the same specific growth rates. In BM, Streptococcus faecalis grew well even without adding each of the sugars tested.
制备了不同聚合度（n = 1 - 6）的N - 乙酰几丁寡糖（(GlcNAc)(n)）作为培养基中的主要碳源，用于评估9种肠道细菌的生长情况。通过用盐酸水解虾壳几丁质制备了几丁质水解产物。纯化后，每种(GlcNAc)(1 - 6)的纯度均>86%。在以0.2%（w/v）的每种糖或葡萄糖作为主要碳源的基础培养基（BM）中进行肠道细菌的生长实验，并使用最大细胞密度和比生长速率进行评估。脆弱拟杆菌和产气荚膜梭菌分别比葡萄糖更有效地利用GlcNAc和(GlcNAc)(2)进行生长。青春双歧杆菌和黏液真杆菌可以略微利用(GlcNAc)(1 - 6)作为它们的主要碳源。大肠杆菌、乳酸乳球菌和普通变形杆菌比(GlcNac)(1 - 6)更有效地利用葡萄糖。金黄色葡萄球菌对GlcNAc的利用比(GlcNAc)(2 - 6)更容易，显示出几乎相同的比生长速率。在BM中，粪肠球菌即使不添加所测试的每种糖也生长良好。
