Wright Lesley C, Chen Sharon C A, Wilson Christabel F, Simpanya Mukoma F, Blackstock Rebecca, Cox Gary M, Murphy Juneann W, Sorrell Tania C
Centre for Infectious Diseases and Microbiology, Level 3, ICPMR Building, Westmead Hospital, Darcy Rd., NSW, 2145, Australia.
FEMS Microbiol Lett. 2002 Apr 9;209(2):175-81. doi: 10.1111/j.1574-6968.2002.tb11128.x.
Extracellular phospholipase (PL) activities comprising phospholipase B, lysophospholipase and lysophospholipase transacylase have been identified in culture supernatants of Cryptococcus neoformans and contribute to virulence. We found that PL production was optimal after fungal growth at 30 degrees C and secretion at 37 degrees C for all six C. neoformans isolates studied (four C. neoformans var. neoformans and two C. neoformans var. gattii). No increase in PL activity was found in one strain, NU-2, in low iron or tissue culture media, conditions where upregulation of other virulence factors has been reported. The most virulent strains in an intravenous mouse model of infection were best able to produce PL at growth and secretion temperatures of 37 degrees C, in tissue culture media and under assay conditions of pH 7.0.
在新型隐球菌的培养上清液中已鉴定出包括磷脂酶B、溶血磷脂酶和溶血磷脂酶转酰基酶在内的细胞外磷脂酶（PL）活性，这些活性有助于其毒力。我们发现，在所研究的所有6株新型隐球菌分离株（4株新型隐球菌新生变种和2株新型隐球菌格特变种）中，真菌在30℃生长并在37℃分泌后，PL的产生最为理想。在低铁或组织培养基中，在已报道其他毒力因子上调的条件下，一株名为NU-2的菌株未发现PL活性增加。在静脉注射小鼠感染模型中，毒力最强的菌株在37℃的生长和分泌温度下、在组织培养基中以及在pH 7.0的测定条件下最能产生PL。
