Kukan M, Vajdová K, Lutterová M, Kristek F, Kebis A, Kuba D, Horecký J
Laboratory of Perfused Organs, Institute of Preventive and Clinical Medicine, Limbová 14, 83301 Bratislava, Slovakia.
Cryobiology. 2001 Dec;43(4):303-9. doi: 10.1006/cryo.2001.2366.
We very recently showed (using a blood-free perfusion model) that cold preservation sensitized rat hepatocyte functions to rewarming ischemic injury and that the injury can be prevented by repleting high-energy adenylates in the liver by short-term oxygenated warm reperfusion. Here we investigated whether short-term reperfusion after the preservation period can improve hepatic graft function in a blood reperfusion model. Eighteen-hour cold-preserved rat livers either untreated (Group A) or pretreated by 30-min oxygenated warm reperfusion after preservation (Group B) were subjected to 20-min ischemic rewarming and then reperfused with blood. Livers in Group B compared to Group A exhibited approx. three times increased bile production and bromosulfophthalein excretion, nearly 7-fold decreased swelling, and 1.2-fold improved blood flow. These results suggest that repletion of the energy by short-term oxygenated reperfusion after prolonged preservation may improve markedly initial hepatic graft function.
我们最近（使用无血灌注模型）表明，冷藏保存会使大鼠肝细胞功能对复温缺血损伤敏感，并且通过短期充氧温血再灌注补充肝脏中的高能腺苷酸可以预防这种损伤。在此，我们研究了保存期后的短期再灌注是否能改善血液再灌注模型中的肝移植功能。将18小时冷藏保存的大鼠肝脏分为两组，一组不做处理（A组），另一组在保存后进行30分钟充氧温血再灌注预处理（B组），然后进行20分钟的缺血复温，随后进行血液再灌注。与A组相比，B组肝脏的胆汁分泌量和溴磺酞排泄量增加了约三倍，肿胀程度降低了近7倍，血流量改善了1.2倍。这些结果表明，长时间保存后通过短期充氧再灌注补充能量可能会显著改善肝移植的初始功能。
