顺序烟酰胺 - 腺嘌呤二核苷酸依赖性氧化还原酶的立体特异性与代谢序列的进化关系。
The stereospecificity of sequential nicotinamide-adenine dinucleotide-dependent oxidoreductases in relation to the evolution of metabolic sequences.
作者信息
do Nascimento K H, Davies D D
出版信息
Biochem J. 1975 Sep;149(3):553-7. doi: 10.1042/bj1490553.
The generalization that 'when a metabolic sequence involves consecutive nicotinamide-adenine dinucleotide-dependent reactions, the dehydrogenases have the same stereospecificity' was tested and confirmed for three metabolic sequences. (1) NAD+-xylitol (D-xylulose) dehydrogenase and NADP+-xylitol (L-xylulose) dehydrogenase are both B-specific. (2) D-Mannitol 1-phosphate dehydrogenase and D-sorbitol 6-phosphate dehydrogenase are both B-specific. (3) meso Tartrate dehydrogenase and oxaloglycollate reductive decarboxylase are both A-specific. Other dehydrogenases associated with the metabolism of meso-tartrate in Pseudomonas putida, such as hydroxypyruvate reductase and tartronate semialdehyde reductase, were also shown to be A-specific. Malate dehydrogenase from Pseudomonas putida was A-specific, and the proposition is discussed that the common A-stereospecificity among the dehydrogenases involved in meso-tartrate metabolism reflects their origin from malate dehydrogenase.
“当一个代谢序列涉及连续的烟酰胺腺嘌呤二核苷酸依赖性反应时，脱氢酶具有相同的立体特异性”这一普遍规律针对三个代谢序列进行了测试并得到证实。（1）NAD⁺-木糖醇（D-木酮糖）脱氢酶和NADP⁺-木糖醇（L-木酮糖）脱氢酶均为B特异性。（2）D-甘露醇1-磷酸脱氢酶和D-山梨醇6-磷酸脱氢酶均为B特异性。（3）内消旋酒石酸脱氢酶和草酰乙醇酸还原脱羧酶均为A特异性。恶臭假单胞菌中与内消旋酒石酸代谢相关的其他脱氢酶，如羟基丙酮酸还原酶和甘油酸半醛还原酶，也被证明是A特异性。恶臭假单胞菌的苹果酸脱氢酶是A特异性，并且讨论了这样一个观点，即参与内消旋酒石酸代谢的脱氢酶之间共同的A立体特异性反映了它们起源于苹果酸脱氢酶。
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