do Nascimento K H, Davies D D
Biochem J. 1975 Sep;149(3):553-7. doi: 10.1042/bj1490553.
The generalization that 'when a metabolic sequence involves consecutive nicotinamide-adenine dinucleotide-dependent reactions, the dehydrogenases have the same stereospecificity' was tested and confirmed for three metabolic sequences. (1) NAD+-xylitol (D-xylulose) dehydrogenase and NADP+-xylitol (L-xylulose) dehydrogenase are both B-specific. (2) D-Mannitol 1-phosphate dehydrogenase and D-sorbitol 6-phosphate dehydrogenase are both B-specific. (3) meso Tartrate dehydrogenase and oxaloglycollate reductive decarboxylase are both A-specific. Other dehydrogenases associated with the metabolism of meso-tartrate in Pseudomonas putida, such as hydroxypyruvate reductase and tartronate semialdehyde reductase, were also shown to be A-specific. Malate dehydrogenase from Pseudomonas putida was A-specific, and the proposition is discussed that the common A-stereospecificity among the dehydrogenases involved in meso-tartrate metabolism reflects their origin from malate dehydrogenase.
“当一个代谢序列涉及连续的烟酰胺腺嘌呤二核苷酸依赖性反应时，脱氢酶具有相同的立体特异性”这一普遍规律针对三个代谢序列进行了测试并得到证实。（1）NAD⁺-木糖醇（D-木酮糖）脱氢酶和NADP⁺-木糖醇（L-木酮糖）脱氢酶均为B特异性。（2）D-甘露醇1-磷酸脱氢酶和D-山梨醇6-磷酸脱氢酶均为B特异性。（3）内消旋酒石酸脱氢酶和草酰乙醇酸还原脱羧酶均为A特异性。恶臭假单胞菌中与内消旋酒石酸代谢相关的其他脱氢酶，如羟基丙酮酸还原酶和甘油酸半醛还原酶，也被证明是A特异性。恶臭假单胞菌的苹果酸脱氢酶是A特异性，并且讨论了这样一个观点，即参与内消旋酒石酸代谢的脱氢酶之间共同的A立体特异性反映了它们起源于苹果酸脱氢酶。
