含沙门氏菌液泡的破坏导致肠炎沙门氏菌鼠伤寒血清型在上皮细胞胞质溶胶中的复制增加。
Disruption of the Salmonella-containing vacuole leads to increased replication of Salmonella enterica serovar typhimurium in the cytosol of epithelial cells.
作者信息
Brumell John H, Tang Patrick, Zaharik Michelle L, Finlay B Brett
机构信息
Biotechnology Laboratory and Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada.
出版信息
Infect Immun. 2002 Jun;70(6):3264-70. doi: 10.1128/IAI.70.6.3264-3270.2002.
Abstract
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that inhabits a vacuolar compartment, called the Salmonella-containing vacuole (SCV), in infected host cells. Maintenance of the SCV is accomplished by SifA, and mutants of this Salmonella pathogenicity island 2 type III effector replicate more efficiently in epithelial cells. Here we demonstrate that enhanced replication of sifA mutants occurs in the cytosol of these cells. Increased replication of wild-type bacteria was also observed in cells treated with wortmannin or expressing Rab5 Q79L or Rab7 N125I, all of which caused a loss of SCV integrity. Our findings demonstrate the requirement of the host cell endosomal system for maintenance of the SCV and that loss of this compartment allows increased replication of serovar Typhimurium in the cytosol of epithelial cells.
摘要
肠炎沙门氏菌鼠伤寒血清型是一种兼性细胞内病原体，它在受感染的宿主细胞中占据一个称为含沙门氏菌液泡（SCV）的液泡区室。SCV的维持由SifA完成，该沙门氏菌致病岛2型III效应器的突变体在上皮细胞中复制效率更高。在这里，我们证明sifA突变体的增强复制发生在这些细胞的细胞质中。在用渥曼青霉素处理或表达Rab5 Q79L或Rab7 N125I的细胞中也观察到野生型细菌的复制增加，所有这些都会导致SCV完整性丧失。我们的研究结果证明了宿主细胞内体系统对维持SCV的必要性，并且该区室的丧失允许鼠伤寒血清型在上皮细胞的细胞质中增加复制。
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