Nakase Takanobu, Sugamoto Kazuomi, Miyamoto Takashi, Tsumaki Noriyuki, Luyten Frank P, Inui Hiroaki, Myoui Akira, Tomita Tetsuya, Yoshikawa Hideki
Department of Orthopaedic Surgery, Osaka University Medical School, 2-2 Yamadaoka, Suita 565-0871, Japan.
Clin Orthop Relat Res. 2002 Jun(399):140-5. doi: 10.1097/00003086-200206000-00016.
Localization and expression of cartilage-derived morphogenetic protein-1 in tissues of torn rotator cuff tendons were examined by in situ hybridization and immunohistochemical analysis. Histologic findings of torn rotator cuff tendons showed that active cells synthesizing the alpha-1 chain of collagen Type I messenger ribonucleic acid were localized predominantly in the torn edge and in the bursa side rather than in the joint side, and scarcely localized in a site distant from the torn edge. Cartilage-derived morphogenetic protein-1 had a similar distribution as the alpha-1 chain of collagen Type I. The current findings provide the first observational evidence that cartilage-derived morphogenetic protein-1 was activated specifically at the site of the torn rotator cuff tendon. The current findings suggest that the cells in the torn rotator cuffs are capable of synthesizing cartilage-derived morphogenetic protein-1, one of the known essential factors for tendon formation.
通过原位杂交和免疫组织化学分析，研究了软骨衍生形态发生蛋白-1在肩袖撕裂肌腱组织中的定位和表达。肩袖撕裂肌腱的组织学结果显示，合成I型胶原蛋白α-1链信使核糖核酸的活性细胞主要定位于撕裂边缘和滑囊侧，而非关节侧，且很少定位于远离撕裂边缘的部位。软骨衍生形态发生蛋白-1的分布与I型胶原蛋白α-1链相似。目前的研究结果提供了首个观察证据，表明软骨衍生形态发生蛋白-1在肩袖撕裂肌腱部位被特异性激活。目前的研究结果表明，肩袖撕裂处的细胞能够合成软骨衍生形态发生蛋白-1，这是已知的肌腱形成必需因子之一。
