吗啡对鸡巯基乙酸盐诱导的腹膜炎的影响。

Majewski Pawel, Markowska Magdalena, Laskowska Hanna, Waloch Maria, Skwarlo-Sonta Krystyna

Department of Vertebrate Physiology, Faculty of Biology, Warsaw University, Poland.

Neuro Endocrinol Lett. 2002 Apr;23(2):161-7.

BACKGROUND

Morphine exerts immunomodulatory effects dependent on several factors including species and parameter examined. The aim of this study was to assess the influence of morphine on experimental peritonitis and leukocyte activity in young chickens of both sexes.

METHODS

Peritonitis was elicited by intraperitoneal injection of thioglycollate (TG) alone or supplemented with morphine; additional chicken groups were injected with morphine alone. Morphine-treated birds (with or without elicited peritonitis) were pretreated with naltrexone, an antagonist of opioid receptors. Control groups were intact or PBS-injected. At specific postinjection intervals peritoneal leukocytes (PTL) were obtained by flushing the peritoneal cavity, counted and used for in vitro assays of activity (respiratory burst). Vascular permeability was measured using Evans blue solution.

RESULTS

Inflammatory reaction and morphine influence were gender-dependent: the extent of TG-induced peritonitis was higher in males and it was additionally stimulated by morphine. This pro-inflammatory effect of morphine was not seen in females. PTL collected from morphine-treated chickens of both sexes exhibited a stronger nitroblue tetrazolium reduction than in non-treated birds and this effect was antagonized by naltrexone.

CONCLUSIONS

The effect of morphine on peritonitis in chickens appears different to that in other vertebrate species, although the mechanism(s) of its influence on leukocyte activity are similar.

背景

吗啡发挥免疫调节作用取决于多种因素，包括所研究的物种和参数。本研究的目的是评估吗啡对年轻雌雄鸡实验性腹膜炎和白细胞活性的影响。

方法

通过腹腔注射单独的巯基乙酸盐（TG）或添加吗啡来诱发腹膜炎；另外的鸡组单独注射吗啡。用阿片受体拮抗剂纳曲酮对接受吗啡治疗的鸡（有或没有诱发腹膜炎）进行预处理。对照组为完整鸡或注射磷酸盐缓冲液（PBS）的鸡。在特定的注射后间隔时间，通过冲洗腹腔获得腹腔白细胞（PTL），计数并用于体外活性测定（呼吸爆发）。使用伊文思蓝溶液测量血管通透性。