Rubio C A
Gastrointestinal and Liver Pathology Research Laboratory, Karolinska Institute and Hospital, Stockholm, Sweden.
Anticancer Res. 2002 Mar-Apr;22(2A):879-81.
Muciphages are mucin-rich phagocytes believed to evolve as a result of the disruption of colorectal crypts. In a previous work we found, in rectal biopsies from patients with chronic ulcerative colitis, muciphages having not only mucin but also lysozyme, an enzyme with a potent antimicrobial activity. Recently we detected lysozyme-rich muciphages in the normal mucosa of the stalk of colonic adenomas. Filed hematoxylin and eosin (H&E)-stained sections from 30 consecutive colorectal adenomas with a stalk (lined by normal colorectal mucosa) were stained with PAS (for mucopolysaccharides), with CD68 (to label macrophages) and with lysozyme (Muramidase). Of the 30 adenomas, 16 (40%) showed muciphages in the mucosa of the stalk. Those muciphages were PAS- CD68- and lysozyme-positive. Although the significance of these findings remains elusive, it is conceivable that lysozyme-rich muciphages mirror increased cell destruction in colorectal adenomas with a high cell turnover. The possibility that lysozyme-rich muciphages surrounding adenomas are instrumental in a novel molecular mechanism of host defense, effective at the early stages of colorectal carcinogenesis, was also entertained. Such a mechanism would prevent the lateral expansion of the dysplastic epithelium of the adenoma into the surrounding normal mucosa of the stalk.
黏液吞噬细胞是富含黏蛋白的吞噬细胞，被认为是结直肠隐窝破坏的结果而进化而来。在之前的一项研究中，我们在慢性溃疡性结肠炎患者的直肠活检组织中发现，黏液吞噬细胞不仅含有黏蛋白，还含有溶菌酶，这是一种具有强大抗菌活性的酶。最近，我们在结肠腺瘤蒂部的正常黏膜中检测到富含溶菌酶的黏液吞噬细胞。对30例连续的带蒂结直肠腺瘤（其蒂部由正常结直肠黏膜衬里）的苏木精和伊红（H&E）染色切片进行过碘酸雪夫染色（PAS，用于检测黏多糖）、CD68染色（标记巨噬细胞）和溶菌酶（ muramidase）染色。在这30例腺瘤中，16例（40%）在蒂部黏膜中显示有黏液吞噬细胞。这些黏液吞噬细胞PAS、CD68和溶菌酶均呈阳性。尽管这些发现的意义尚不清楚，但可以想象，富含溶菌酶的黏液吞噬细胞反映了细胞更新率高的结直肠腺瘤中细胞破坏增加的情况。我们还考虑了腺瘤周围富含溶菌酶的黏液吞噬细胞在一种新的宿主防御分子机制中发挥作用的可能性，这种机制在结直肠癌发生的早期阶段是有效的。这样一种机制将阻止腺瘤发育异常的上皮细胞向周围蒂部正常黏膜的侧向扩展。
