Kiziridou A D, Toliou T, Stefanou D, Agnantis N
Department of Pathology, Theagenion Anticancer Institute of Thessaloniki, Greece.
Anticancer Res. 2002 Mar-Apr;22(2A):985-90.
The malignant potential of solid tumors is related to their ability to invade adjacent tissue and to metastasize. The plasminogen activation system is one of the critical factors in tumor progression since it is involved in tumor invasion and metastasis. This study was performed to examine the expression of u-PA in benign, borderline and malignant tumors of the ovary by immunohistochemical evaluation on formalin-fixed, paraffin-embedded specimens applying monoclonal antibody 3689 directed to the b-chain of u-PA. Normal epithelial cells of the ovary (n = 5) showed no staining of u-PA but some stromal cells were slightly stained. Invasive carcinomas (n = 16) and borderline tumors (n = 15) showed a moderate to strong diffuse cytoplasmic staining. Benign tumors (n = 20) showed a variety of staining. The observation of randomly positive u-PA stromal cells is noteworthy. The percentage of u-PA-positive tumors was higher in carcinomas than in other tumors. There was no correlation with other known risk factors of malignancy such as differentiation, stage or type of tumor. In conclusion there are noticeable differences in u-PA expression among ovarian tumors and u-PA increase in ovarian tumors can be attributed to an increased diffuse cytoplasmic content in the neoplastic epithelial cells.
实体瘤的恶性潜能与其侵袭邻近组织和发生转移的能力有关。纤溶酶原激活系统是肿瘤进展的关键因素之一，因为它参与肿瘤侵袭和转移。本研究通过应用针对尿激酶型纤溶酶原激活剂（u-PA）b链的单克隆抗体3689，对福尔马林固定、石蜡包埋标本进行免疫组织化学评估，以检测u-PA在卵巢良性、交界性和恶性肿瘤中的表达。卵巢正常上皮细胞（n = 5）未显示u-PA染色，但一些基质细胞有轻微染色。浸润性癌（n = 16）和交界性肿瘤（n = 15）显示中度至强的弥漫性细胞质染色。良性肿瘤（n = 20）显示出多种染色情况。值得注意的是观察到随机出现的u-PA阳性基质细胞。u-PA阳性肿瘤的百分比在癌中高于其他肿瘤。与其他已知的恶性风险因素如肿瘤分化、分期或类型无关。总之，卵巢肿瘤之间u-PA表达存在显著差异，卵巢肿瘤中u-PA的增加可归因于肿瘤上皮细胞中弥漫性细胞质含量的增加。
