用内源性结肠有丝分裂抑制剂焦谷氨酸-组氨酸-甘氨酸处理的HT-29细胞中早期癌基因mRNA的表达。
Early oncogene mRNA expression in HT-29 cells treated with the endogenous colon mitosis inhibitor pyroglutamyl-histidyl-glycine.
作者信息
Reichelt Wenche H, Liu Ying, Luna Luisa, Eigjo Kiell, Reichelt Karl L
机构信息
Institute of Pediatric Research, University of Oslo, The National Hospital, Norway.
出版信息
Anticancer Res. 2002 Mar-Apr;22(2A):991-6.
BACKGROUND
The tripeptide pyroGlu-His-GlyOH (pEHG), isolated from intestinal extracts (1), suppresses growth of human colonic epithelial cells and human colorectal adenocarcinoma cells (HT-29) both in vitro and in vivo. The present study represents the first attempt to relate alterations in relevant gene expression to the effect on cell growth.
MATERIALS AND METHODS
Northern blot with RNA, extracted from HT-29 cells exposed to pEHG, was hybridised with c-fos and c-myc probes.
RESULTS
c-fos gene expression was doubled in HT-29 cells after 20 minutes of incubation with pEHG and decreased to half the initial value after 2 hours. The increase at 20 minutes was concentration-dependent at a peptide concentration range from 10(-6)-10(-12) M. The expression of the oncogene c-myc showed only marginal alternations at the concentrations and times tested.
CONCLUSION
The colon mitosis-inhibiting peptide pEHG increases gene expression of c-fos, but not that of c-myc.
背景
从肠道提取物中分离出的三肽焦谷氨酸-组氨酸-甘氨酸（pEHG），在体外和体内均能抑制人结肠上皮细胞和人结肠直肠腺癌细胞（HT-29）的生长。本研究首次尝试将相关基因表达的变化与对细胞生长的影响联系起来。
材料与方法
用从暴露于pEHG的HT-29细胞中提取的RNA进行Northern印迹，并用c-fos和c-myc探针杂交。
结果
用pEHG孵育20分钟后，HT-29细胞中c-fos基因表达增加一倍，2小时后降至初始值的一半。在10(-6)-10(-12) M的肽浓度范围内，20分钟时的增加呈浓度依赖性。在测试的浓度和时间下，癌基因c-myc的表达仅显示出轻微变化。
结论
结肠有丝分裂抑制肽pEHG增加c-fos的基因表达，但不增加c-myc的基因表达。
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