Wan Suigui, Gong Wenyu, Sun Xuejing, Xu Juan, Tian Ding
Department of Hematology, Xuanwu Hospital, CUMS, Beijing 100053, China.
Zhonghua Xue Ye Xue Za Zhi. 2002 Feb;23(2):83-6.
To evaluate the specificity of three-color flow cytometry in childhood acute lymphoblastic leukemia (ALL) immunophenotyping.
Immunophenotyping was performed by three-color flow cytometry analysis using CD(45)/SSC gating.
The percentage of blasts was correlated better with leukemic cell count compared with that of FSC/SSC, and the false positive results were low. Among eighty six cases of ALL, 95.3% was B-ALL, in which common-ALL and Pro-B-ALL were 76.8% and 6.1%, respectively, and 2.3% was T-ALL. CD(34)(+) and myeloid-associated antigen expression were observed in 57.0% and 34.9% of the cases, respectively, among which Pro-B-ALL was the commonest. CD(33) was more commonly expressed than CD(13) in Pro-B-ALL cases, but no difference in the expression between these two antigens in other subtypes.
Gating of CD(45)/SSC eliminated effection of normal cells to blasts in bone marrow, with which the immunophenotyping results were more reliable.
评估三色流式细胞术在儿童急性淋巴细胞白血病（ALL）免疫表型分析中的特异性。
采用CD(45)/SSC设门法通过三色流式细胞术分析进行免疫表型分析。
与FSC/SSC相比，原始细胞百分比与白血病细胞计数的相关性更好，假阳性结果较低。在86例ALL病例中，95.3%为B-ALL，其中普通ALL和前B-ALL分别占76.8%和6.1%，2.3%为T-ALL。分别在57.0%和34.9%的病例中观察到CD(34)(+)和髓系相关抗原表达，其中前B-ALL最为常见。在前B-ALL病例中，CD(33)比CD(13)更常表达，但在其他亚型中这两种抗原的表达无差异。
CD(45)/SSC设门消除了正常细胞对骨髓中原始细胞的影响，由此免疫表型分析结果更可靠。
