Liang Yingmin, Sun Qiang, Jiang Shanshan, Wang Jizhu, Wu Rongli, Chen Ping, Liu Li, Han Hua
Department of Hematology of Tangdu Hospital and Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi'an 710038, China.
Zhonghua Xue Ye Xue Za Zhi. 2002 Jan;23(1):5-8.
To construct a vector containing protein transduction domain (PTD) and bcr/abl fusion gene of chronic myelogenous leukemia and express PTD-bcr/abl fusion protein in E. Coli.
DNA fragment encoding PTD was synthesized and fused to PCR-amplified bcr/abl gene fragment, then inserted into plasmid pET-16b to get the expression vector pEPb containing PTD-bcr/abl fusion gene, which was transfected and expressed in E. Coli LB21. PTD-bcr/abl fusion protein was purified by affinity chromatography.
523 bp bcr/abl fusion gene was effectively amplified. The PTD-bcr/abl gene sequencing showed the same sequence as scheduled. The fusion peptide was successfully expressed in E. Coli and purified.
The results may provide a new PTD-bcr/abl fusion peptide for the immunotherapy of CML.
