Jones G A, Taylor A
Can J Microbiol. 1975 Nov;21(11):1803-6. doi: 10.1139/m75-262.
Inocula of rumen fluid from sheep were grown on a medium of low nitrogen content under an oxygen-free atmosphere containing N2. The cultures produced methane and carbon dioxide. Hydrogen gas was utilized and the partial pressure of N2 decreased by 24% in 48 h. Assuming this N2 to have been reduced to metabolically available nitrogenous compounds, these account for about 0.2% of the sheep's daily need.
将来自绵羊的瘤胃液接种物在含N₂的无氧气氛下，于低氮含量培养基上培养。培养物产生了甲烷和二氧化碳。氢气被利用，48小时内N₂的分压降低了24%。假设这些N₂已被还原为代谢可用的含氮化合物，这些化合物约占绵羊每日需求量的0.2%。
