Ribeiro Carlos, Ebner Andreas, Affolter Markus
Abteilung Zellbiologie, Biozentrum der Universität Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
Dev Cell. 2002 May;2(5):677-83. doi: 10.1016/s1534-5807(02)00171-5.
In the developing tracheal system of Drosophila melanogaster, six major branches arise by guided cell migration from a sac-like structure. The chemoattractant Branchless/FGF (Bnl) appears to guide cell migration and is essential for the formation of all tracheal branches, while Decapentaplegic (Dpp) signaling is strictly required for the formation of a subset of branches, the dorsal and ventral branches. Using in vivo confocal video microscopy, we find that the two signaling systems affect different cellular functions required for branching morphogenesis. Bnl/FGF signaling affects the formation of dynamic filopodia, possibly controlling cytoskeletal activity and motility as such, and Dpp controls cellular functions allowing branch morphogenesis and outgrowth.
在黑腹果蝇发育中的气管系统中，六个主要分支通过引导细胞从一个囊状结构迁移而产生。趋化因子无分支/成纤维细胞生长因子（Bnl）似乎引导细胞迁移，并且对于所有气管分支的形成至关重要，而骨形态发生蛋白（Dpp）信号传导对于一部分分支，即背侧和腹侧分支的形成是严格必需的。利用体内共聚焦视频显微镜，我们发现这两种信号系统影响分支形态发生所需的不同细胞功能。Bnl/FGF信号传导影响动态丝状伪足的形成，可能由此控制细胞骨架活性和运动性，而Dpp控制允许分支形态发生和生长的细胞功能。
