体内成像揭示了成纤维细胞生长因子（FGF）和二聚体骨形态发生蛋白（Dpp）信号在气管分支形态发生中的不同细胞功能。

In vivo imaging reveals different cellular functions for FGF and Dpp signaling in tracheal branching morphogenesis.

作者信息

Ribeiro Carlos, Ebner Andreas, Affolter Markus

机构信息

Abteilung Zellbiologie, Biozentrum der Universität Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.

出版信息

Dev Cell. 2002 May;2(5):677-83. doi: 10.1016/s1534-5807(02)00171-5.

DOI:10.1016/s1534-5807(02)00171-5

PMID:12015974

Abstract

In the developing tracheal system of Drosophila melanogaster, six major branches arise by guided cell migration from a sac-like structure. The chemoattractant Branchless/FGF (Bnl) appears to guide cell migration and is essential for the formation of all tracheal branches, while Decapentaplegic (Dpp) signaling is strictly required for the formation of a subset of branches, the dorsal and ventral branches. Using in vivo confocal video microscopy, we find that the two signaling systems affect different cellular functions required for branching morphogenesis. Bnl/FGF signaling affects the formation of dynamic filopodia, possibly controlling cytoskeletal activity and motility as such, and Dpp controls cellular functions allowing branch morphogenesis and outgrowth.

摘要

在黑腹果蝇发育中的气管系统中，六个主要分支通过引导细胞从一个囊状结构迁移而产生。趋化因子无分支/成纤维细胞生长因子（Bnl）似乎引导细胞迁移，并且对于所有气管分支的形成至关重要，而骨形态发生蛋白（Dpp）信号传导对于一部分分支，即背侧和腹侧分支的形成是严格必需的。利用体内共聚焦视频显微镜，我们发现这两种信号系统影响分支形态发生所需的不同细胞功能。Bnl/FGF信号传导影响动态丝状伪足的形成，可能由此控制细胞骨架活性和运动性，而Dpp控制允许分支形态发生和生长的细胞功能。