中心粒周围高尔基体复合体的碎片化和分散是哺乳动物细胞进入有丝分裂所必需的。
Fragmentation and dispersal of the pericentriolar Golgi complex is required for entry into mitosis in mammalian cells.
作者信息
Sütterlin Christine, Hsu Pattie, Mallabiabarrena Arrate, Malhotra Vivek
机构信息
Cell and Developmental Biology Department, Division of Biology, University of California, San Diego, La Jolla 92093, USA.
出版信息
Cell. 2002 May 3;109(3):359-69. doi: 10.1016/s0092-8674(02)00720-1.
The pericentriolar Golgi stacks are fragmented and found dispersed in mitotic mammalian cells. Addition of an antibody to the Golgi-associated protein GRASP65 inhibited Golgi fragmentation by mitotic cytosol in permeabilized cells. Microinjecting this antibody or the C-terminal fragment of GRASP65, which contains the antibody binding site, into normal rat kidney cells prevented entry into mitosis. Under these conditions the cells had completed S phase but were not in the prophase stage of mitosis. Fragmentation of the Golgi apparatus by nocodazole or Brefeldin A treatment prior to or post microinjection of the anti-GRASP65 antibody alleviated the block in mitotic entry. Based on our findings, we suggest that the pericentriolar Golgi organization is a sensor for controlling entry into mitosis in mammalian cells.
中心粒周围的高尔基体堆叠在有丝分裂的哺乳动物细胞中发生片段化并分散分布。向通透细胞中添加针对高尔基体相关蛋白GRASP65的抗体可抑制有丝分裂细胞质导致的高尔基体片段化。将该抗体或包含抗体结合位点的GRASP65 C末端片段显微注射到正常大鼠肾细胞中可阻止细胞进入有丝分裂。在这些条件下，细胞已完成S期，但未处于有丝分裂前期。在显微注射抗GRASP65抗体之前或之后，用诺考达唑或布雷菲德菌素A处理使高尔基体碎片化，可缓解有丝分裂进入的阻滞。基于我们的研究结果，我们认为中心粒周围的高尔基体组织是控制哺乳动物细胞进入有丝分裂的一种传感器。
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