Nilsson I, Utt M
Division of Bacteriology, Department of Medical Microbiology, Dermatology and Infection (MMDI), University of Lund, Solvegatan 23, S-223 62 Lund, Sweden.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 May 5;771(1-2):251-60. doi: 10.1016/s1570-0232(02)00113-7.
The analysis of Helicobacter pylori proteins is a demanding task for the elucidation of virulence factors, antigens and vaccines, all important for diagnosis, therapy and protection. In the "pre-genomic era" the purification of proteins was mostly performed by using various techniques such as detergent treatment of the bacterial cells, ultra-centrifugation, various chromatographic methods, antibody detection, N-terminal sequence determination and finally cloning and identification of the corresponding gene. In this review, the most representative methods used for purification, separation and identification of H. pylori proteins will be presented as well as some important developments in the "post-genomic era" that have improved the performance of these characterisation techniques.
对幽门螺杆菌蛋白质进行分析，对于阐明毒力因子、抗原和疫苗来说是一项艰巨的任务，而这些对于诊断、治疗和预防都至关重要。在“基因组学时代之前”，蛋白质的纯化大多通过使用各种技术来进行，如对细菌细胞进行去污剂处理、超速离心、各种色谱方法、抗体检测、N端序列测定，最后进行相应基因的克隆和鉴定。在这篇综述中，将介绍用于纯化、分离和鉴定幽门螺杆菌蛋白质的最具代表性的方法，以及“基因组学时代之后”一些改进了这些表征技术性能的重要进展。
