McClellan M C, Dieckman M A, Abel J H, Niswender G D
Cell Tissue Res. 1975 Dec 10;164(3):291-307. doi: 10.1007/BF00223011.
The development of granulosa-lutein cells was studied in 27 normal and 32 superovulated ewes between days 0-4(day 0 began with the preovulatory LH peak in normal animals and the HCG injection in superovulated ewes). The pattern of differentiation was similar in both groups. Following initial hormonal stimulation (0-12 hours after LH or HCG), granulosa cells were approximately 100 mu2 and contained small, pleomorphic nuclei with large amounts of clumped chromatin. Elongate cells lining the basement membrane possessed large, heterogeneous dense bodies, and a well-developed Golgi apparatus. Mitotic figures were observed up to 6 hours prior to ovulation. Sixteen to 20 hours following the LH surge or HCG injection, hypertrophy of granulosa cells was evident. Nuclei contained definitive nucleoli. Blood vessels in the theca interna were abundant and highly dilated. Ovulation occurred approximately 24 hours after the LH peak or HCG injection. Visible signs of luteinization were evident 6-12 hours after ovulation. A slight increase in serum progesterone levels was detected. The second post-ovulatory day was characterized by continuing hypertrophy of granulosa cells and extensive proliferation of smooth endoplasmic reticulum and mitochondria. Nuclei of granulosa cells were larger and possessed extremely large nucleoli. Numerous mitotic figures were apparent within the corpus luteum. Serum progesterone concentrations began increasing at 60-72 hours after hormone stimulation. By the end of the third post-ovulatory day, the corpus luteum consisted of large, pleomorphic, parenchymal cells, interspersed between capillaries and connective tissue elements. Only an occasional mitotic figure was apparent within the corpus luteum at 100 hours. Light microscopic autoradiography of 5, 10, and 15 day corpora lutea taken from ewes pulsed with 3H thymidine at specific times before and after ovulation revealed that granulosa cells did not undergo secondary mitoses following ovulation. In contrast, thecal, mesenchymal and endothelial cells did mitose on day 3.
对27只正常母羊和32只超排母羊在第0 - 4天（正常动物以排卵前促黄体生成素峰开始计算第0天，超排母羊以注射人绒毛膜促性腺激素开始计算第0天）期间的颗粒黄体细胞发育情况进行了研究。两组的分化模式相似。在初始激素刺激后（促黄体生成素或人绒毛膜促性腺激素注射后0 - 12小时），颗粒细胞面积约为100μm²，含有小的、多形性核，伴有大量聚集的染色质。衬于基底膜的细长细胞具有大的、异质性致密小体以及发育良好的高尔基体。在排卵前6小时仍可观察到有丝分裂象。促黄体生成素峰或注射人绒毛膜促性腺激素后16 - 20小时，颗粒细胞肥大明显。细胞核含有明确的核仁。内膜中的血管丰富且高度扩张。排卵在促黄体生成素峰或注射人绒毛膜促性腺激素后约24小时发生。排卵后6 - 12小时可见黄体化的明显迹象。检测到血清孕酮水平略有升高。排卵后第二天的特征是颗粒细胞持续肥大，滑面内质网和线粒体广泛增殖。颗粒细胞的细胞核更大，具有极大的核仁。黄体内部可见大量有丝分裂象。激素刺激后60 - 72小时血清孕酮浓度开始升高。到排卵后第三天结束时，黄体由大的、多形性实质细胞组成，散布于毛细血管和结缔组织成分之间。在100小时时，黄体内部仅偶尔可见有丝分裂象。对在排卵前后特定时间用³H胸腺嘧啶核苷脉冲处理的母羊的第5、10和15天黄体进行光镜放射自显影显示，排卵后颗粒细胞未进行二次有丝分裂。相比之下，膜细胞、间充质细胞和内皮细胞在第3天进行了有丝分裂。
