Fichna P, Malendowicz L K
Cell Tissue Res. 1975 Dec 10;164(3):411-24. doi: 10.1007/BF00223018.
Adult male rats were treated daily for 18 days with endoxan in doses of 4 mg/kg of body weight or testosterone in doses 2.5 mg per rat. For the last 5 days of the experiment some of the rats received gonadotrophin injections in doses of 50 I.U. Karyometric and stereologic studies were undertaken on paraffin sections of the testes stained with haematoxylin and eosin. Endoxan treatment decrease the nuclear volume of the Leydig cells and lowers the volume fraction occupied by the interstitial gland in the testis. These changes were accompanied by a decrease in the intensity of reaction for acid phosphatase, non-specific esterases and 3-beta-hydroxy-steroid dehydrogenase. Testosterone injected alone or jointly with endoxan resulted in more pronounced changes than following treatment with endoxan alone. As expected, gonadotrophin injections into intact rats resulted in a marked increase in nuclear volume of the Leydig cells and in the volume fraction of the interstitial gland. This effect of gonadotrophin on the interstitial gland of the rat testis was partially inhibited by endoxan treatment. It follows from these experiments that endoxan impairs the functional activity of the interstitial gland of the rat testes by lowering the endogenous gonadotrophin levels, as well as by direct action on the Leydig cells.
成年雄性大鼠每天接受环磷酰胺（剂量为4毫克/千克体重）或睾酮（剂量为每只大鼠2.5毫克）处理，持续18天。在实验的最后5天，一些大鼠接受了剂量为50国际单位的促性腺激素注射。对用苏木精和伊红染色的睾丸石蜡切片进行了细胞核测量和体视学研究。环磷酰胺处理降低了睾丸间质细胞的核体积，并降低了睾丸间质腺所占的体积分数。这些变化伴随着酸性磷酸酶、非特异性酯酶和3-β-羟基类固醇脱氢酶反应强度的降低。单独注射睾酮或与环磷酰胺联合注射所导致的变化比单独用环磷酰胺处理更为明显。正如预期的那样，向未处理的大鼠注射促性腺激素导致睾丸间质细胞的核体积和间质腺的体积分数显著增加。环磷酰胺处理部分抑制了促性腺激素对大鼠睾丸间质腺的这种作用。从这些实验可以看出，环磷酰胺通过降低内源性促性腺激素水平以及直接作用于睾丸间质细胞，损害了大鼠睾丸间质腺的功能活性。
