Dai R, Wang X, Bi K, Luo X
Shenyang Pharmaceutical University, Shenyang 110015.
Yao Xue Xue Bao. 1998 Sep;33(9):707-9.
The steps of statistical simulation spectrophotometry are to build a model based on a collection of limited but sufficient data from simulation samples to reveal the real absorbance-concentration relationship of the real sample and provide concentrated data of the individual components of the real sample measured by its absorbance data at several sensitive wavelengths. In this article, the method of stepwise regression has been used for building absorbance-concentration model of Feibaosanxiao tablets, a compound formulation of multimedicaments, and uniform design, for experimental design and recovery test. Recoveries of the four medicaments, acetaminophen, phenytoin sodium, theophylline and caffeine were 99.6%, 97.4%, 100.7% and 100.7% with RSD 0.3%, 1.9%, 1.3% and 1.3% respectively (n = 14).
统计模拟分光光度法的步骤是基于来自模拟样品的有限但足够的数据集合建立模型，以揭示真实样品的实际吸光度-浓度关系，并通过其在几个敏感波长处的吸光度数据提供真实样品中各成分的浓缩数据。本文采用逐步回归法建立了多药复方制剂肺宝三效片的吸光度-浓度模型，并采用均匀设计进行实验设计和回收率试验。对乙酰氨基酚、苯妥英钠、茶碱和咖啡因四种药物的回收率分别为99.6%、97.4%、100.7%和100.7%，相对标准偏差分别为0.3%、1.9%、1.3%和1.3%（n = 14）。
