Yan C, Feng Y
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050.
Yao Xue Xue Bao. 1998 Jul;33(7):486-92.
The effects of l-3-n-butylphthalide(l-NBP) and d-3-n-butylphthalide(d-NBP) on hypoxia/hypoglycemia-induced cytotoxicity in primary cultured rat cortical neurons were studied. l-NBP and d-NBP(1-100 mumol.L-1) were shown to inhibit hypoxia/hypoglycemia-induced LDH release, decrease the percent of cell death and improve the damaged cellular morphology at 10 mumol.L-1 concentration. In addition, l-NBP, d-NBP and dl-NBP were also found to significantly reduce the liberation of polyribosomes from the neuronal rough endoplasmic reticulum and disaggregation of polyribosomes induced by hypoxia/hypoglycemia. These data suggest that l-NBP, d-NBP and dl-NBP can remarkably protect cultured neurons against hypoxia/hypoglycemia induced damage.
研究了左旋-3-正丁基苯酞（l-NBP）和右旋-3-正丁基苯酞（d-NBP）对原代培养大鼠皮层神经元缺氧/低血糖诱导的细胞毒性的影响。结果显示，l-NBP和d-NBP（1-100μmol·L-1）在10μmol·L-1浓度时可抑制缺氧/低血糖诱导的乳酸脱氢酶（LDH）释放，降低细胞死亡率，并改善受损的细胞形态。此外，还发现l-NBP、d-NBP和消旋-3-正丁基苯酞（dl-NBP）能显著减少缺氧/低血糖诱导的多核糖体从神经元粗面内质网的释放以及多核糖体的解聚。这些数据表明，l-NBP、d-NBP和dl-NBP能显著保护培养的神经元免受缺氧/低血糖诱导的损伤。
