Xu H L, Feng Y P
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
Zhongguo Yao Li Xue Bao. 1999 Oct;20(10):929-33.
The effects of dl-3-n-butylphthalide (dl-NBP), l-3-n-butylphthalide (l-NBP), and d-3-n-butylphthalide (d-NBP) on the production of nitric oxide (NO), epoprostenol (Epo) and endothelin-1 (ET-1) were investigated in cerebrovascular and aortic endothelium in culture.
Bovine cerebral endothelial cells (BCEC) and bovine aortic endothelial cells (BAEC) were cultured in Medium 199 in vitro. After incubation with dl-, l-, and d-NBP for 24 h, the release of NO, Epo, and ET-1 were analyzed by using spectrometry assay and radioimmunoassay (RIA) respectively.
Low concentrations of dl- and l-NBP (0.1-10 mumol.L-1) enhanced nitrite and 6-ketoprostaglandin F1 alpha (6-ketoPGF1 alpha) production in both BAEC and BCEC after a 24-h incubation, and l-NBP has a potent effect on promoting Epo production in BCEC. The production of ET-1 secreted by BCEC and BAEC was increased after TNF alpha stimulation, this enhancement was not blunted by the simultaneous addition of dl-, l-, and d-NBP.
研究消旋-3-正丁基苯酞（dl-NBP）、左旋-3-正丁基苯酞（l-NBP）和右旋-3-正丁基苯酞（d-NBP）对培养的脑血管和主动脉内皮细胞中一氧化氮（NO）、依前列醇（Epo）和内皮素-1（ET-1）生成的影响。
牛脑内皮细胞（BCEC）和牛主动脉内皮细胞（BAEC）在体外199培养基中培养。用dl-NBP、l-NBP和d-NBP孵育24小时后，分别采用光谱分析法和放射免疫分析法（RIA）分析NO、Epo和ET-1的释放情况。
低浓度的dl-NBP和l-NBP（0.1-10μmol·L-1）孵育24小时后，可增强BAEC和BCEC中亚硝酸盐和6-酮前列腺素F1α（6-ketoPGF1α）的生成，且l-NBP对促进BCEC中Epo的生成有显著作用。TNFα刺激后，BCEC和BAEC分泌的ET-1生成增加，同时添加dl-NBP、l-NBP和d-NBP并不会减弱这种增强作用。
1）dl-NBP和l-NBP可增加BCEC和BAEC中NO的生成。2）l-NBP增加BCEC中Epo生成的作用比在BAEC中更明显，且dl-NBP对增加BCEC中Epo的生成具有选择性作用。
