Rui J, Zhou X, Lin S, Yuan Y, Xiang B, An D
Department of Clinical Pharmacology, Jinling Hospital, Nanjing 210002.
Yao Xue Xue Bao. 1998 Jul;33(7):517-22.
An analytical method has been developed to determine simultaneously plasma concentration of the enantiomers of verapamil and its major metabolite, norverapamil, using capillary zone electrophoresis. Trimethyl-beta-cyclodextrin was selected as chiral selector. Good separation was achieved under the conditions as below: Running electrolyte was pH 2.5, 60 mmol.L-1 phosphate buffer containing 60 mmol.L-1 trimethyl-beta-cyclodextrin; capillary: 30 cm x 75 microns (ID), coated; setpoint of capillary temperature: 20 degrees C; detector: UV 200 nm; injection: electromigration at 12 kV for 7 s; running voltage: 14 kV. Solvent of sample was doubly deionized water. The migration times of enantiomers were less than 12 min. The good linear range was from 300.0 ng.ml-1 to 2.50 ng.ml-1. Relative standard deviations of assay accuracy and precision were less than 12%. It fits for routine therapeutic monitoring as it is highly efficient, rapid, sensitive, with less solvent and sample consumption and automation.
已开发出一种分析方法，使用毛细管区带电泳同时测定维拉帕米对映体及其主要代谢物去甲维拉帕米的血浆浓度。选择三甲基-β-环糊精作为手性选择剂。在以下条件下实现了良好的分离：运行电解质为pH 2.5、含有60 mmol.L-1三甲基-β-环糊精的60 mmol.L-1磷酸盐缓冲液；毛细管：30 cm×75微米（内径），涂层；毛细管温度设定点：20℃；检测器：UV 200 nm；进样：在12 kV下电迁移7 s；运行电压：14 kV。样品溶剂为双去离子水。对映体的迁移时间小于12分钟。良好的线性范围为300.0 ng.ml-1至2.50 ng.ml-1。分析准确度和精密度的相对标准偏差小于12%。它高效、快速、灵敏，溶剂和样品消耗少且自动化，适用于常规治疗监测。
