Xiong J, Feng Y
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050.
Yao Xue Xue Bao. 1998 Jun;33(6):401-6.
Transient cerebral ischemia may cause striking changes in gene expression in rat brain. The induction of heat shock protein 70 (hsp70) mRNA is considered to be an important marker of cerebral ischemia injury, and c-fos may upregulate the expression of other genes related to the secondary injuries. dl-3-n-Butylphthaline(NBP) had been shown to have good anti-cerebral ischemic effect. Using the in situ hybridization and Northern blot technique, the effect of NBP on the expression of hsp70 mRNA and c-fos in transient middle cerebral artery occlusion (MCAo) rat caused by intraluminal thread was studied, and found that the expression of hsp70 mRNA was at the lesioned site at 1 h of reperfusion. It increased gradually with the duration of reperfusion time and peaked at 12 h at the lesioned site. With NBP treatment(i.p. 10 mg.kg-1 10 min before ischemia or 20 mg.kg-1 after ischemia), the expression of hsp70 mRNA attenuated significantly. For c-fos, the expression appeared at 0.5 h of reperfusion, peaked at 3 h, and decreased at 6 h. NBP pretreatment (10 mg.kg-1 10 min before ischemia) also decreased the c-fos expression. The same results were obtained with Northern blot technique. Since NBP had been shown to have good anti-cerebral ischemic effects, the attenuating effect on gene expression seemed to be the secondary effect after the alleviation of tissue injury.
短暂性脑缺血可能导致大鼠脑内基因表达发生显著变化。热休克蛋白70（hsp70）mRNA的诱导被认为是脑缺血损伤的一个重要标志物，而c-fos可能上调与继发性损伤相关的其他基因的表达。已证明dl-3-正丁基苯酞（NBP）具有良好的抗脑缺血作用。采用原位杂交和Northern印迹技术，研究了NBP对由管腔内丝线造成的短暂性大脑中动脉闭塞（MCAo）大鼠hsp70 mRNA和c-fos表达的影响，发现再灌注1小时时，hsp70 mRNA在损伤部位表达。其随再灌注时间的延长而逐渐增加，并在损伤部位于再灌注12小时达到峰值。用NBP治疗（缺血前10分钟腹腔注射10 mg·kg-1或缺血后20 mg·kg-1），hsp70 mRNA的表达显著减弱。对于c-fos，其表达在再灌注0.5小时出现，3小时达到峰值，6小时下降。NBP预处理（缺血前10分钟10 mg·kg-1）也降低了c-fos的表达。Northern印迹技术也得到了相同的结果。由于已证明NBP具有良好的抗脑缺血作用，对基因表达的减弱作用似乎是组织损伤减轻后的继发效应。
