Aberrant expression of cyclin A in laryngeal squamous cell carcinoma.

The expression of cyclin A protein was retrospectively investigated in 102 patients with human laryngeal squamous cell carcinoma (LSCC) by using immunohistochemistry. Out of 102 tumors, 41 (40.2%) exhibited positive-staining in cancer cells with this antibody. No association between cyclin A overexpression and the patients' clinicopathological parameters was found. However, cyclin A overexpression was significantly associated with CDK2 and PCNA expression (p = 0.01 and p = 0.001, respectively). Kaplan-Meier analysis showed that patients with overexpression of cyclin A were associated with poor disease-free survival (p = 0.046), but it was not associated with overall survival (p = 0.106). Additionally, patients with both lymph node metastasis and cyclin A overexpression were significantly associated with worse disease-free survival (p < 0.0001). The multivariate analysis showed that tumor size, lymph node metastasis and cyclin A overexpression are significantly independent prognostic factors of disease-free survival. These findings suggest that cyclin A overexpression is associated with recurrence of LSCC.