Grushko O G, Sharakhov I V, Stegniĭ V N
Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, 634050 Russia.
Genetika. 2002 Apr;38(4):483-8.
Chromosome localization of sequences homologous to lambda 20p1.4 of the Drosophila melanogaster nuclear lamina DNA (nlDNA) was established by in situ hybridization in species of the melanogaster subgroup. DNA of the lambda 20p1.4 clone was shown to be located in the chromocenter in all the species examined. Laboratory strains of D. simulans, D. mauritiana, and D. sechellia exhibited interspecific differences in localization of lambda 20p1.4 nlDNA on chromosome arms. In eight natural populations, intraspecific polymorphism of lambda 20p1.4 nlDNA chromosome localization was shown to be present in D. simulans but absent in D. melanogaster. The possible participation of transposable elements in nlDNA relocation is discussed.
通过在黑腹果蝇亚组物种中的原位杂交,确定了与黑腹果蝇核纤层DNA(nlDNA)的λ20p1.4同源序列的染色体定位。λ20p1.4克隆的DNA在所有检测的物种中均位于染色中心。拟暗果蝇、毛里求斯果蝇和塞舌尔果蝇的实验室菌株在λ20p1.4 nlDNA在染色体臂上的定位表现出种间差异。在八个自然种群中,拟暗果蝇存在λ20p1.4 nlDNA染色体定位的种内多态性,而黑腹果蝇中不存在。讨论了转座元件在nlDNA重新定位中可能的参与情况。