Sharakhov I V, Bogachev S S, Fisher P A
Genetika. 1997 Feb;33(2):277-80.
M/SAR of Drosophila melanogaster DNA clone gamma 20p 1.4 was localized on chromosomes by in situ hybridization of the gamma 20p 1.4 DNA fragment and its deletion mutants with salivary gland chromosomes of third instar larvae of the lines Canton S and Oregon R. The M/SAR sequence, capable of specifically binding to lamin in vitro, was detected in the pericentric regions 20CD, 40EF, 41AB, the proximal part of region 81 of chromosome 3, and region 101 of chromosome 4, which attach to the nuclear envelope in cells of D. melanogaster salivary glands, and in region 49D, which does not come into contact with the nuclear periphery. This is the first M/SAR localized in pericentric chromatin of D. melanogaster.
通过将果蝇黑腹果蝇DNA克隆γ20p 1.4的DNA片段及其缺失突变体与Canton S和Oregon R品系三龄幼虫的唾液腺染色体进行原位杂交,将其M/SAR定位在染色体上。在果蝇黑腹果蝇唾液腺细胞中与核膜相连的20CD、40EF、41AB的着丝粒周围区域、3号染色体81区的近端部分和4号染色体101区,以及不与核周边接触的49D区,检测到了能够在体外与核纤层蛋白特异性结合的M/SAR序列。这是首次在果蝇黑腹果蝇着丝粒染色质中定位的M/SAR。