Chernin J, Thompson D, Swaine D
School of Biological Sciences, University of Portsmouth, Hants, UK.
J Helminthol. 2002 Mar;76(1):21-5. doi: 10.1079/joh2001102.
Tetrathyridia of Mesocestoides corti were cultured in vitro in a diphasic medium consisting of a liquid medium (CMRL Sigma) and a thixotropic nutrient gel (Oxoid). Tests demonstrated that a 50% medium/gel mixture produced optimum conditions for the survival and development of tetrathyridia. Established anthelminthic drugs were inoculated into the gel which demonstrated that this system can be used for preliminary anthelminthic drug screening. The development and survival of the tetrathyridia were influenced by the addition of pepsin, trypsin and liver peptone to the culture media. The development and maturation of proglottids were observed in addition to asexual reproduction by the process of budding. Tetrathyridia maintained in vitro and reinfected into both mouse and rat hosts retained their viability.
将科氏中殖孔绦虫的四槽蚴在由液体培养基（CMRL Sigma）和触变营养凝胶（Oxoid）组成的双相培养基中进行体外培养。试验表明，50%的培养基/凝胶混合物为四槽蚴的存活和发育创造了最佳条件。将已有的驱虫药接种到凝胶中，结果表明该系统可用于初步的驱虫药筛选。向培养基中添加胃蛋白酶、胰蛋白酶和肝蛋白胨会影响四槽蚴的发育和存活。除了通过出芽过程进行无性繁殖外，还观察到了节片的发育和成熟。在体外维持并重新感染小鼠和大鼠宿主的四槽蚴保持了它们的活力。
