Yamada Tomiaki, Fatigati Michael A, Zhang Min
JGC, Bio-Refinery Group, New Business Development Operations, No. 2 Project Division, Yokohama, Japan.
Appl Biochem Biotechnol. 2002 Spring;98-100:899-907. doi: 10.1385/abab:98-100:1-9:899.
By applying the Arkenol process using highly concentrated sulfuric acid, various biomass feedstocks, including cedar tree, rice straw, newspaper, and bagasse, were successfully processed and converted into glucose and xylose for fermentation usage in a flash fermentation reactor in which the performance of National Renewable Energy Laboratory's patented rec-Zymomonas mobilis 31821 (pZB5) after immobilization was investigated. The immobilization medium is a photocrosslinked resin made from polyethylene glycols or polypropylene glycols. Recombinant or rec-Z. mobilis used in the study has been shown to efficiently ferment glucose and xylose at a relatively high concentration (12-15%), that is a typical hydrolysate produced from cellulosic feedstocks. The application of immobilized rec-Z. mobilis and flash fermentation technology, together with concentrated acid technology producing a high concentration sugar solution, promises to speed the development of the cellulose-to-ethanol industry.
通过应用使用高浓度硫酸的Arkenol工艺，包括雪松、稻草、报纸和甘蔗渣在内的各种生物质原料被成功加工，并在闪速发酵反应器中转化为葡萄糖和木糖用于发酵，在此反应器中对美国国家可再生能源实验室的专利重组运动发酵单胞菌31821（pZB5）固定化后的性能进行了研究。固定化介质是由聚乙二醇或聚丙二醇制成的光交联树脂。研究中使用的重组或rec-Z. mobilis已被证明能够在相对高的浓度（12-15%）下高效发酵葡萄糖和木糖，这是纤维素原料产生的典型水解产物。固定化rec-Z. mobilis和闪速发酵技术的应用，以及生产高浓度糖溶液的浓酸技术，有望加速纤维素制乙醇产业的发展。
