Ahmed G, Roy P K, Mamun A N
Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, AERE, Dhaka, Bangladesh.
Indian J Exp Biol. 2001 Dec;39(12):1322-4.
Shoot tip and nodal segment explants of Holarrhena antidysenterica when cultured on MS medium containing BAP (1.0-3.0 mg/l) with NAA (0.2-1.0 mg/l) and BAP (1.0-3.0 mg/l) with Kn. (0.2-1.0 mg/l) produced multiple shoots. Maximum multiple shoots was found in MS medium supplemented with BAP (2.0 mg/l) and NAA (0.5 mg/l). Subculture on the same medium resulted in rapid shoot multiplication at an average rate of 16 new shoots per subculture. Addition of urea (100 mg/l) in the medium increased the number of shoots up to 22 per culture. For best rooting, the shoots were excised from the culture flask and implanted individually on half strength MS medium with 0.5 mg/l each of IBA, IAA and NAA. After 20 days of transfer on root induction medium 95% rooting was achieved. Regenerated plantlets were successfully acclimatized and established in soil. About 90% of plantlets survived under open field conditions.
止泻木（Holarrhena antidysenterica）的茎尖和节段外植体在含有1.0 - 3.0毫克/升苄氨基嘌呤（BAP）与0.2 - 1.0毫克/升萘乙酸（NAA）以及1.0 - 3.0毫克/升BAP与0.2 - 1.0毫克/升激动素（Kn）的MS培养基上培养时，能产生多个芽。在添加了2.0毫克/升BAP和0.5毫克/升NAA的MS培养基中发现了最多的多个芽。在相同培养基上继代培养导致芽快速增殖，平均每次继代培养有16个新芽。在培养基中添加100毫克/升尿素使每个培养物中的芽数增加到22个。为了实现最佳生根，将芽从培养瓶中切下，单独接种在含有0.5毫克/升吲哚丁酸（IBA）、吲哚乙酸（IAA）和萘乙酸（NAA）的1/2强度MS培养基上。转移到生根诱导培养基20天后，生根率达到95%。再生的小植株成功驯化并在土壤中定植。在露天条件下，约90%的小植株存活。
