Deodhar Swapna R, Thengane R J, Thengane S R
Plant Tissue Culture Division, National Chemical Laboratory, Pune 411008, India.
Indian J Exp Biol. 2008 Jun;46(6):482-6.
Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 microM) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root segments produced lesser shoots. Shoots (0.5-0.8 cm) were transferred to elongation medium consisting of Woody Plant Medium (WPM) supplemented with BAP (4.44-22.69 microM), IAA (5.71 microM) and kinetin (4.65 microM). It was observed that shoot length increased to 1-2 cm. WPM medium supplemented with NAA (2.69-10.74 microM) and IBA (4.90 microM) induced rooting within 20-25 days. Using the present protocol, 20-25 plantlets could be regenerated from single root explant within 3 to 4 months. The protocol has potential for large scale production of elite plants.
印度藤黄幼苗的根在添加了BAP（0.44 - 2.22微摩尔）的半强度MS培养基上长期培养时，会产生新的芽。与母株相连的根产生的芽更多，而切下的根段产生的芽较少。将芽（0.5 - 0.8厘米）转移到由添加了BAP（4.44 - 22.69微摩尔）、IAA（5.71微摩尔）和激动素（4.65微摩尔）的木本植物培养基（WPM）组成的伸长培养基上。观察到芽的长度增加到1 - 2厘米。添加了NAA（2.69 - 10.74微摩尔）和IBA（4.90微摩尔）的WPM培养基在20 - 25天内诱导生根。使用本方案，在3至4个月内，单个根外植体可再生出20 - 25株幼苗。该方案具有大规模生产优良植株的潜力。
