Manju R V, Kulkarni M J, Prasad T G, Sudarshana L, Sashidhar V R
Department of Crop Physiology, University of Agricultural Sciences, Bangalore, India.
Indian J Exp Biol. 2001 Aug;39(8):786-92.
Contents of trans-zeatin riboside (ZR), dihydrozeatin riboside (DZR) and N6-(delta2-isopentenyl) adenosine (iPA) was quantified by an indirect ELISA using polyclonal antibodies, in the roots, xylem sap and leaves of pot grown sunflower plants subjected to water stress (RWC of leaves approximately 65 per cent). The delivery rates of all three cytokinins decreased significantly under stress. Cytokinin levels also decreased in roots and in leaves of stressed plants. Three-fold increase in cytokinin oxidase activity was observed in stressed roots after polymin P-ammonium sulphate fractionation. Further purification using Con A agarose resulted in elution of protein with cytokinin oxidase activity and was found to be 30 kDa protein on SDS-PAGE.
通过使用多克隆抗体的间接酶联免疫吸附测定法（ELISA），对遭受水分胁迫（叶片相对含水量约为65%）的盆栽向日葵植株的根、木质部汁液和叶片中的反式玉米素核苷（ZR）、二氢玉米素核苷（DZR）和N6-（Δ2-异戊烯基）腺苷（iPA）含量进行了定量分析。在胁迫条件下，所有三种细胞分裂素的转运速率均显著下降。胁迫植株的根和叶中的细胞分裂素水平也降低。经聚明胶肽-硫酸铵分级分离后，在胁迫根中观察到细胞分裂素氧化酶活性增加了三倍。使用伴刀豆球蛋白A琼脂糖进一步纯化，得到具有细胞分裂素氧化酶活性的蛋白质洗脱物，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上发现其为30 kDa的蛋白质。
