Tango M S A, Ghaly A E
Department of Biological Engineering, Dalhousie University, P.O. Box 1000, Halifax, Nova Scotia, B3J 2X4, Canada.
Appl Microbiol Biotechnol. 2002 May;58(6):712-20. doi: 10.1007/s00253-002-0970-3. Epub 2002 Mar 23.
A 5 l packed bed bioreactor was used to study the effect of initial lactose concentration and hydraulic retention time (HRT) on cell growth, lactose utilization and lactic acid production. Up to 95% of the initial lactose concentration was utilized at longer HRTs (30-36 h). The study showed that lactic acid production increased with increases in HRT (12-36 h) and initial lactose concentrations. The highest lactic acid production rate (3.90 g l(-1) h(-1)) was obtained with an initial lactose concentration of 100 g/l and an HRT of 18 h, whereas the lowest lactic acid production rate (1.35 g l(-1) h(-1)) was obtained with an initial lactose concentration of 50 g/l and an HRT of 36 h. This suggested that optimal lactic acid production can be achieved at an HRT of 18 h and initial lactose concentration of 100 g/l.
使用一个5升的填充床生物反应器来研究初始乳糖浓度和水力停留时间（HRT）对细胞生长、乳糖利用和乳酸产生的影响。在较长的水力停留时间（30 - 36小时）下，高达95%的初始乳糖浓度被利用。研究表明，乳酸产量随着水力停留时间（12 - 36小时）和初始乳糖浓度的增加而增加。当初始乳糖浓度为100 g/l且水力停留时间为18小时时，获得了最高的乳酸产生速率（3.90 g l(-1) h(-1)），而当初始乳糖浓度为50 g/l且水力停留时间为36小时时，获得了最低的乳酸产生速率（1.35 g l(-1) h(-1)）。这表明在水力停留时间为18小时且初始乳糖浓度为100 g/l时可以实现最佳的乳酸产生。
