脂肪酸在兔胎盘的转运。

Transfer of fatty acids across the rabbit placenta.

作者信息

Elphick M C, Hudson D G, Hull D

出版信息

J Physiol. 1975 Oct;252(1):29-42. doi: 10.1113/jphysiol.1975.sp011132.

DOI:10.1113/jphysiol.1975.sp011132

PMID:1202197

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1348466/

Abstract

Transfer of fatty acids across the placenta was studied in anaesthetized rabbits at 28-days gestation by measuring umbilical venous-arterial differences, by injection of labelled palmitate into the mother and observing its appearance in the foetus, by injection of labelled palmitate into the foetus and measuring its appearance in the mother and the foetal clearance rate. 2. The release of fatty acids and glycerol by foetal adipose tissues was investigated in vitro by measuring the effect of addition of noradrenaline to the incubation medium and in vivo by measuring the effect of noradrenaline infusion into the foetus on circulating glycerol and free fatty acid concentrations. 3. In anaesthetized rabbits at 28-days gestation the maternal circulating free fatty acid concentrations were high and there was a positive umbilical venous-arterial difference. High maternal free fatty acid concentrations were associated with high umbilical venous-arterial differences. 4. Label was present in the foetus in 2 min and reached a peak in 3 min after injection of labelled palmitate into the mother. Label appeared in the maternal circulation in 1 min after injection into the foetus. The half-life of labelled palmitate was of the order of 30-60 sec in both mother in foetus. 5. Foetal white adipose tissue released both free fatty acids and glycerol into the medium and the rate of release increased four to five fold after addition of noradrenaline. Infusion of noradrenaline in the foetus led to a rise in glucose and glycerol concentrations, but the change in free fatty acid concentrations was not significant. 6. It was concluded that (i) free fatty acids can cross the rabbit placenta in amounts sufficient to provide the fatty acid components of stored triglyceride and structural lipids; (ii) placental transport of free fatty acids depends in part on maternal blood concentration and on foetal uptake; (iii) foetal circulating free fatty acids are continually exchanging with fatty acid pools in the placenta and with the maternal circulating free fatty acids.

摘要

通过测量脐静脉 - 动脉差异、向母体注射标记的棕榈酸盐并观察其在胎儿中的出现情况、向胎儿注射标记的棕榈酸盐并测量其在母体中的出现情况以及胎儿清除率，对妊娠28天的麻醉兔进行了脂肪酸跨胎盘转运的研究。2. 通过测量向孵育培养基中添加去甲肾上腺素的效果，在体外研究了胎儿脂肪组织释放脂肪酸和甘油的情况；通过测量向胎儿输注去甲肾上腺素对循环甘油和游离脂肪酸浓度的影响，在体内进行了研究。3. 在妊娠28天的麻醉兔中，母体循环游离脂肪酸浓度较高，脐静脉 - 动脉存在正向差异。母体游离脂肪酸浓度高与脐静脉 - 动脉差异大相关。4. 向母体注射标记的棕榈酸盐后2分钟，标记物出现在胎儿体内，并在3分钟达到峰值。向胎儿注射后1分钟，标记物出现在母体循环中。标记的棕榈酸盐在母体和胎儿中的半衰期约为30 - 60秒。5. 胎儿白色脂肪组织向培养基中释放游离脂肪酸和甘油，添加去甲肾上腺素后释放速率增加了四到五倍。向胎儿输注去甲肾上腺素导致葡萄糖和甘油浓度升高，但游离脂肪酸浓度变化不显著。6. 得出的结论是：(i) 游离脂肪酸能够以足以提供储存甘油三酯和结构脂质的脂肪酸成分的量穿过兔胎盘；(ii) 游离脂肪酸的胎盘转运部分取决于母体血液浓度和胎儿摄取；(iii) 胎儿循环游离脂肪酸不断与胎盘中的脂肪酸池以及母体循环游离脂肪酸进行交换。