反复暴露于+Gz后大鼠脑内HSP70基因的表达
Expression of HSP70 gene in rat brain after exposures to repeated +Gz.
作者信息
Cai Q, Liu H J, Chen Y C, Ji G Y, Chen T X
机构信息
Molecular Biology Research Center, General Hospital of The Air Force, Beijing.
出版信息
Space Med Med Eng (Beijing). 1999 Oct;12(5):313-7.
OBJECTIVE
To study the changes of mRNA expression of heat shock protein 70 (HSP70) in the rat brain exposed to repeated +Gz.
METHOD
The mRNA expression levels of HSP70 in rat brain were measured by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).
RESULT
The HSP70 mRNA expression levels in rat brains taken 30 min and 6 h after repeated +Gz exposures were significantly higher than those in control group, while the difference between the levels of control group and those of experimental rat brains taken 24 h after +Gz exposure was not significant.
CONCLUSION
It is suggested that HSP70 mRNA expression in rat brain can be induced by repeated +Gz exposures and the increased HSP70 mRNA expression may play an important role in self-protection against brain damage induced by +Gz exposures.
目的
研究反复暴露于+Gz环境下大鼠脑内热休克蛋白70（HSP70）mRNA表达的变化。
方法
采用半定量逆转录聚合酶链反应（RT-PCR）检测大鼠脑内HSP70的mRNA表达水平。
结果
反复暴露于+Gz环境30分钟和6小时后大鼠脑内HSP70 mRNA表达水平显著高于对照组，而+Gz暴露24小时后实验组大鼠脑内HSP70 mRNA表达水平与对照组相比差异无统计学意义。
结论
提示反复暴露于+Gz环境可诱导大鼠脑内HSP70 mRNA表达增加，且HSP70 mRNA表达增加可能在对抗+Gz暴露所致脑损伤的自我保护中起重要作用。
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