Thaung Caroline, Arnold Karen, Jackson Ian J, Coffey Peter J
MRC Mammalian Genetics Unit, Harwell OX11 0RD, UK.
Neurosci Lett. 2002 May 31;325(1):21-4. doi: 10.1016/s0304-3940(02)00223-9.
We have generated a large number of potential eye and vision mutants by a mouse mutagenesis programme and sought to develop a rapid method for testing vision. This experiment assesses whether the visual tracking drum can differentiate between mice with and without retinal degeneration. The population of mice tested had a proportion with retinal degeneration, the remainder having normal retinas. Mice were observed in the visual tracking drum for a head tracking response to a visual stimulus. They were then examined for retinal degeneration. Of 204 animals tested, 138 had retinal degeneration. None of these showed any head tracking response. Of the remaining 66 with normal retinas, 45 showed a normal response, and 19 of the 21 mice which did not respond on a first test showed a normal response when the test was repeated. The visual tracking drum differentiates reliably between mice with normal vision and mice with severely decreased vision. There was a relatively high rate of non-responders among the normal sighted mice on a first test, but the test is rapid enough to be repeated easily.
我们通过一项小鼠诱变计划生成了大量潜在的眼部和视觉突变体，并试图开发一种快速测试视觉的方法。本实验评估视觉跟踪鼓能否区分患有和未患有视网膜变性的小鼠。受试小鼠群体中一部分患有视网膜变性，其余小鼠视网膜正常。在视觉跟踪鼓中观察小鼠对视觉刺激的头部跟踪反应。然后检查它们是否患有视网膜变性。在测试的204只动物中，138只患有视网膜变性。这些动物均未表现出任何头部跟踪反应。其余66只视网膜正常的小鼠中，45只表现出正常反应，在首次测试中未做出反应的21只小鼠中有19只在重复测试时表现出正常反应。视觉跟踪鼓能够可靠地区分视力正常的小鼠和视力严重下降的小鼠。在首次测试中，视力正常的小鼠中无反应者的比例相对较高，但该测试速度足够快，可以轻松重复进行。
