Reis Rodrigo A, Tischer Cesar A, Gorin Philip A J, Iacomini Marcello
Departamento de Bioquímica, Universidade Federal do Paraná, C.P. 19046, 81531-990, Curitiba, Brazil.
FEMS Microbiol Lett. 2002 Apr 23;210(1):1-5. doi: 10.1111/j.1574-6968.2002.tb11152.x.
The polysaccharides formed on hot alkaline extraction of the ascomycetous lichen Teloschistes flavicans were fractionated to give two glucans, which were characterised by methylation analysis and 1D and 2D NMR spectroscopy. One was a branched beta-glucan containing (1-->3) and (1-->6) linkages, a structure which is more typical of basidiomycetes rather than ascomycetes, which have linear glucans. The other was an alpha-glucan with alternating (1-->4) and (1-->6) linkages, found for the first time in Nature. This structure can be classified as a pullulan, which has been isolated from the fungi Aureobasidium pullulans, Tremella mesenterica, and Cyttaria harioti, but has different ratios of the component glycosidic linkages. The significance of the presence of the isolated alpha- and beta-glucans is discussed.
对从子囊地衣黄网衣（Teloschistes flavicans）经热碱提取得到的多糖进行分级分离，得到了两种葡聚糖，通过甲基化分析以及一维和二维核磁共振光谱对其进行了表征。一种是含有（1→3）和（1→6）连接的分支β-葡聚糖，这种结构在担子菌中更为典型，而非具有线性葡聚糖的子囊菌。另一种是具有交替（1→4）和（1→6）连接的α-葡聚糖，这是首次在自然界中发现。这种结构可归类为支链淀粉，支链淀粉已从出芽短梗霉（Aureobasidium pullulans）、金黄银耳（Tremella mesenterica）和哈氏鸟巢菌（Cyttaria harioti）中分离得到，但各组成糖苷键的比例不同。文中讨论了分离得到的α-和β-葡聚糖存在的意义。
