Tanaka Junji, Tutumi Yutaka, Li Zhang, Katho Naoko, Sugita Junichi, Mori Akio, Toyoshima Nobuyasu, Ota Shuichi, Kobayashi Masanobu, Kasai Masaharu, Asaka Masahiro, Imamura Masahiro
Department of Haematology and Oncology, Graduate School of Medicine, N15 W7, Kita-ku, Sapporo 060-8638, Japan.
Br J Haematol. 2002 Jun;117(3):751-4. doi: 10.1046/j.1365-2141.2002.03525.x.
We report the increased expression of inhibitory natural killer (NK) cell receptor, CD94/NKG2A, for human leucocyte antigen (HLA) class I on CD8+ T cells in granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cells (G-PBMC) after mixed lymphocyte culture (MLC). The addition of purified CD14+ cells to CD14-depleted G-PBMC-induced CD94/NKG2A expression in a dose-dependent fashion; however, this enhancing effect was inhibited by the membrane between responder cells and CD14+ cells. Therefore, CD14+ cells in G-PBMC induce CD94/NKG2A expression on CD8+ T cells, which in turn appear to downregulate alloresponses after allogeneic peripheral blood stem cell transplantation.
我们报告了在混合淋巴细胞培养（MLC）后，粒细胞集落刺激因子动员的外周血单个核细胞（G-PBMC）中，CD8⁺T细胞上抑制性自然杀伤（NK）细胞受体CD94/NKG2A对人类白细胞抗原（HLA）I类分子的表达增加。将纯化的CD14⁺细胞添加到去除CD14的G-PBMC中，以剂量依赖的方式诱导CD94/NKG2A表达；然而，这种增强作用被反应细胞与CD14⁺细胞之间的膜所抑制。因此，G-PBMC中的CD14⁺细胞诱导CD8⁺T细胞上CD94/NKG2A的表达，这反过来似乎会下调异基因外周血干细胞移植后的同种异体反应。
