Song Bao-Hua, Wang Xiao-Quan, Wang Xiao-Ru, Sun Lan-Ju, Hong De-Yuan, Peng Pei-Hao
Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Mol Ecol. 2002 Jun;11(6):1057-63. doi: 10.1046/j.1365-294x.2002.01502.x.
Previous morphological, allozyme and chloroplast DNA data have suggested that Pinus densata originated through hybridization between P. tabuliformis and P. yunnanensis. In the present study, sequence and restriction site analyses of maternally inherited mitochondrial nad1 intron were used to detect variation patterns in 19 populations of P. tabuliformis, P. yunnanensis and P. densata. A total of three mitotypes (A, B, C) were detected. All but one of the populations of P. yunnanensis possessed mitotype B while all populations of P. tabuliformis had mitotype A. Pinus densata populations, on the other hand, harboured both mitotypes A and B, which are characteristic of P. tabuliformis and P. yunnanensis, respectively. This result gives strong additional evidence supporting the hybrid origin of this diploid pine. The distribution of mitotypes indicated very different mating compositions and evolutionary history among P. densata populations. It seems that local founder populations and backcrosses may have played important roles in the early establishment of P. densata populations. The uplift of the Tibetan Plateau had a significant impact on the distribution of maternal lineages of P. densata populations.
先前的形态学、等位酶和叶绿体DNA数据表明，高山松是油松和云南松杂交产生的。在本研究中，对母系遗传的线粒体nad1内含子进行序列和限制性位点分析，以检测油松、云南松和高山松19个种群的变异模式。共检测到三种线粒体类型（A、B、C）。除一个云南松种群外，其他所有云南松种群都具有线粒体类型B，而所有油松种群都具有线粒体类型A。另一方面，高山松种群同时拥有线粒体类型A和B，分别是油松和云南松的特征。这一结果为支持这种二倍体松树的杂交起源提供了有力的额外证据。线粒体类型的分布表明高山松种群之间的交配组成和进化历史非常不同。看来当地的奠基种群和回交可能在高山松种群的早期建立中发挥了重要作用。青藏高原的隆升对高山松种群母系谱系的分布产生了重大影响。
