Watson Fiona, Kiernan Rachel S, Dimaline Rod
The Department of Pathology, Duncan Building, The University of Liverpool, Daulby Street, Liverpool L69 3GA, UK.
Biochim Biophys Acta. 2002 Jun 7;1576(1-2):198-202. doi: 10.1016/s0167-4781(02)00301-9.
In this study, we used the gastric epithelial cell line AGS-G(R) to investigate the role of GATA transcription factors in the regulation of both basal and gastrin-stimulated L-histidine decarboxylase (HDC) gene transcription. Using reporter gene technology, we compared the transcriptional activity of a construct, hHDC503, which contained the 5'-flanking region of the human HDC gene with that of similar constructs lacking selected GATA consensus sequences. We demonstrated the expression of GATA-4 and GATA-6 proteins within the AGS-G(R) cells and found evidence that these transcription factors can negatively regulate HDC gene expression.
