Berzas Nevado J J, Castañeda Peñalvo G, Pinilla Calderón M J
Department of Analytical Chemistry and Food Technology, Faculty of Chemistry, University of Castilla-La Mancha, E-13071 Ciudad Real, Spain.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Jun 25;773(2):151-8. doi: 10.1016/s1570-0232(02)00139-3.
The use of micellar electrokinetic chromatography for the determination of lormetazepam and its metabolite, lorazepam, in serum samples at a concentration range of therapeutic interest was investigated. The separation was carried out at 30 degrees C and 25 kV, using a 15 mM borate-phosphate buffer (pH 8) with 30 mM sodium dodecyl sulfate as the separation electrolyte and 15% methanol as organic modifier. The analyses were carried out in 20 min under these conditions. Detection limits of 0.5 mg l(-1) were achieved for both benzodiazepines in serum. This method was employed for the quantitative resolution of both drugs (at different concentration ratios) in serum with very good recoveries.
